Argonaute on Maragkakis Labhttp://maragkakislab.com/tags/argonaute/Recent content in Argonaute on Maragkakis LabHugoen-usMon, 01 Jan 2018 00:00:00 +0000cCLIP-Seq: Retrieval of Chimeric Reads from HITS-CLIP (CLIP-Seq) Librarieshttp://maragkakislab.com/publications/2018-cclip-seq/Mon, 01 Jan 2018 00:00:00 +0000http://maragkakislab.com/publications/2018-cclip-seq/<h2 id="summary">Summary</h2> <p>This methods chapter describes cCLIP-seq, a computational approach for identifying and retrieving chimeric reads from HITS-CLIP libraries, where a small RNA and its target RNA are co-ligated and sequenced as a single molecule. By resolving these chimeric tags, cCLIP-seq enables direct identification of small RNA binding sites and precise base-pairing profiles at single-nucleotide resolution.</p>