Summary This study introduces a method for transcript-guided targeted enrichment of specific cell populations prior to single-nucleus RNA sequencing. By selecting nuclei based on expression of specific transcripts, the approach achieves deep profiling of rare cell types at scale, enabling discovery of cell-type-specific transcriptional programs that would be missed by unbiased approaches.
Summary This study combines single-cell analysis with long-read nanopore sequencing to capture full-length RNA isoforms and uncover temporal changes in RNA transcription, processing, and alternative splicing in the aging mouse cortex and hippocampus.
Summary TERA-Seq is a method that combines chemical ligation of adapters to both RNA ends with nanopore direct RNA sequencing to capture complete native RNA molecules from the 5’ cap to the 3’ poly(A) tail. This enables simultaneous characterization of RNA modifications, poly(A) tail length, and transcript boundaries at single-molecule resolution.