http://maragkakislab.com/tags/nanopore-sequencing/Recent content in Nanopore Sequencing on Maragkakis LabHugoen-usFri, 13 Oct 2023 00:00:00 +0000http://maragkakislab.com/publications/2023-polya-tail-nanopore/Fri, 13 Oct 2023 00:00:00 +0000http://maragkakislab.com/publications/2023-polya-tail-nanopore/<h2 id="summary">Summary</h2> <p>This methods chapter describes a protocol for transcriptome-wide measurement of differential poly(A) tail lengths using nanopore direct RNA sequencing, combined with linear mixed models for statistical analysis. The approach enables quantitative comparison of poly(A) tail dynamics across conditions and provides a framework for studying the role of deadenylation in post-transcriptional gene regulation.</p>