Nanopore

Full-length direct RNA sequencing uncovers stress granule-dependent RNA decay upon cellular stress

Summary Using full-length direct RNA sequencing, this study uncovers a previously unrecognized role for stress granules in promoting selective mRNA decay during cellular stress. The findings show that mRNAs recruited to stress granules undergo preferential degradation, linking stress granule biology to RNA homeostasis.

Deep learning and direct sequencing of labeled RNA captures transcriptome dynamics

Summary We developed RNAkinet, a neural network that identifies newly synthesized RNA molecules labeled with 5-ethynyl uridine (5EU) using nanopore direct RNA sequencing, processing raw electrical signals without requiring basecalling or sequence alignment. RNAkinet enables simultaneous analysis of RNA metabolism alongside poly(A) tail length and RNA modifications at single-molecule resolution.

TERA-Seq: True end-to-end sequencing of native RNA molecules for transcriptome characterization

Summary TERA-Seq is a method that combines chemical ligation of adapters to both RNA ends with nanopore direct RNA sequencing to capture complete native RNA molecules from the 5’ cap to the 3’ poly(A) tail. This enables simultaneous characterization of RNA modifications, poly(A) tail length, and transcript boundaries at single-molecule resolution.