RNA-Binding Proteins on Maragkakis Lab

Mis-spliced transcripts generate de novo proteins in TDP-43-related ALS/FTD

Fri, 26 Jan 2024 00:00:00 +0000

Summary

This study demonstrates that loss of TDP-43 function leads to widespread cryptic splicing events that produce novel protein isoforms not normally expressed in healthy cells. These de novo proteins may contribute to the pathogenesis of ALS and FTD by triggering immune responses or impairing cellular functions.

RNA-mediated control of protein translation under stress

Thu, 20 Jul 2023 00:00:00 +0000

Summary

This review covers the post-transcriptional mechanisms by which cells regulate protein synthesis in response to stress, focusing on the roles of RNA-binding proteins, non-coding RNAs, and mRNA modifications in controlling translation. The connections between stress-responsive translational control and aging are discussed.

CLIPSeqTools — a novel bioinformatics CLIP-seq analysis suite

Fri, 01 Jan 2016 00:00:00 +0000

Summary

CLIPSeqTools is a modular bioinformatics pipeline for the analysis of CLIP-seq data, providing tools for read processing, peak calling, motif analysis, and visualization. The suite enables comprehensive characterization of RNA-binding protein interaction sites across the transcriptome.

FUS regulates genes coding for RNA-binding proteins in neurons by binding to their highly conserved introns

Mon, 01 Apr 2013 00:00:00 +0000

Summary

CLIP-seq analysis reveals that FUS, an ALS-associated RNA-binding protein, preferentially binds to highly conserved intronic sequences of genes encoding other RNA-binding proteins in neurons. This autoregulatory network suggests a mechanism by which FUS dysfunction could broadly disrupt neuronal RNA metabolism.

Identification of in vivo, conserved, TAF15 RNA binding sites reveals the impact of TAF15 on the neuronal transcriptome

Thu, 21 Feb 2013 00:00:00 +0000

Summary

Using CLIP-seq, this study identifies the in vivo binding sites of TAF15, an ALS-associated RNA-binding protein, across the neuronal transcriptome. The findings reveal evolutionarily conserved binding sites and provide insight into the role of TAF15 in neuronal RNA metabolism.