Single-Cell

Summary This study introduces a method for transcript-guided targeted enrichment of specific cell populations prior to single-nucleus RNA sequencing. By selecting nuclei based on expression of specific transcripts, the approach achieves deep profiling of rare cell types at scale, enabling discovery of cell-type-specific transcriptional programs that would be missed by unbiased approaches.

Summary This study combines single-cell analysis with long-read nanopore sequencing to capture full-length RNA isoforms and uncover temporal changes in RNA transcription, processing, and alternative splicing in the aging mouse cortex and hippocampus.