TERA-Seq: True end-to-end sequencing of native RNA molecules for transcriptome characterization

Summary

TERA-Seq is a method that combines chemical ligation of adapters to both RNA ends with nanopore direct RNA sequencing to capture complete native RNA molecules from the 5’ cap to the 3’ poly(A) tail. This enables simultaneous characterization of RNA modifications, poly(A) tail length, and transcript boundaries at single-molecule resolution.